Vol 59, No 10 (2003)
Other
Published online: 2005-12-12
Submitted: 2012-12-28
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In vitro photodynamic diagnosis of atherosclerotic wall changes with the use of mono-l-aspartyl chlorin e6. A preliminary report

Dariusz Biały, Arkadiusz Derkacz, Magdalena Wawrzyńska, Artur Bednarkiewicz, Piotr Ziółkowski, Halina Nowosad, Wiesław Stręk
Kardiol Pol 2003;59(10):298-301.
Vol 59, No 10 (2003)
Other
Published online: 2005-12-12
Submitted: 2012-12-28

Abstract

Background: Although several methods for atherosclerosis detection are available, none of them seems to be accurate enough to identify the vulnerable atheromatous plaque. Photodynamic diagnosis (PDD) and therapy (PDT) - a new method evaluated for neoplasm treatment, is a modern approach for detecting and treating atherosclerosis.
Aim: To asses in vitro the capability of PDD with the use of chlorin e6 to detect atherosclerotic plaque and the usefulness of this method as a feedback system for photoangioplasty treatment.
Methods: 30 specimens of human aorta and 15 specimens of human coronary arteries were examined. The samples were soaked with chlorin e6 and then washed out. The luminescence spectra were then collected. All samples were examined with light microscopy.
Results: Tissue fluorescence is seen as green light. We noted a very strong red fluorescence of chlorin e6 originating from lipid-rich plaque. We established a quantitative factor (R) which is the ratio of chlorin e6 red intensity in its 660 nm maximum to the area of green luminescence centred at 515 nm. The highest value of R was reached at the atheromatous samples, followed by calcified and normal ones R2=3.51±0.62, R3=1.63±0.31, and R1=1.51±0.15, respectively. A statistically significant difference was noted between groups two and one, and between groups two and three (R2=3.51±0.62 vs. R3=1.63±0.31, p<0.05; and R2=3.51±0.62 vs. R1=1.51±0.15, p<0.05, respectively).
Conclusions: This in vitro study confirms that photosensitiser chlorin e6 accumulates within atheromatous plaque. It may be a specific tool for atheromatous and normal or calcified segments discrimination. The advantage of the above method is the possibility of a real-time imaging followed by targeted therapy of various forms and stages of atherosclerosis.

Abstract

Background: Although several methods for atherosclerosis detection are available, none of them seems to be accurate enough to identify the vulnerable atheromatous plaque. Photodynamic diagnosis (PDD) and therapy (PDT) - a new method evaluated for neoplasm treatment, is a modern approach for detecting and treating atherosclerosis.
Aim: To asses in vitro the capability of PDD with the use of chlorin e6 to detect atherosclerotic plaque and the usefulness of this method as a feedback system for photoangioplasty treatment.
Methods: 30 specimens of human aorta and 15 specimens of human coronary arteries were examined. The samples were soaked with chlorin e6 and then washed out. The luminescence spectra were then collected. All samples were examined with light microscopy.
Results: Tissue fluorescence is seen as green light. We noted a very strong red fluorescence of chlorin e6 originating from lipid-rich plaque. We established a quantitative factor (R) which is the ratio of chlorin e6 red intensity in its 660 nm maximum to the area of green luminescence centred at 515 nm. The highest value of R was reached at the atheromatous samples, followed by calcified and normal ones R2=3.51±0.62, R3=1.63±0.31, and R1=1.51±0.15, respectively. A statistically significant difference was noted between groups two and one, and between groups two and three (R2=3.51±0.62 vs. R3=1.63±0.31, p<0.05; and R2=3.51±0.62 vs. R1=1.51±0.15, p<0.05, respectively).
Conclusions: This in vitro study confirms that photosensitiser chlorin e6 accumulates within atheromatous plaque. It may be a specific tool for atheromatous and normal or calcified segments discrimination. The advantage of the above method is the possibility of a real-time imaging followed by targeted therapy of various forms and stages of atherosclerosis.
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Keywords

atheromatous plaque - photodynamic diagnosis - photodynamic therapy

About this article
Title

In vitro photodynamic diagnosis of atherosclerotic wall changes with the use of mono-l-aspartyl chlorin e6. A preliminary report

Journal

Kardiologia Polska (Polish Heart Journal)

Issue

Vol 59, No 10 (2003)

Pages

298-301

Published online

2005-12-12

Bibliographic record

Kardiol Pol 2003;59(10):298-301.

Keywords

atheromatous plaque - photodynamic diagnosis - photodynamic therapy

Authors

Dariusz Biały
Arkadiusz Derkacz
Magdalena Wawrzyńska
Artur Bednarkiewicz
Piotr Ziółkowski
Halina Nowosad
Wiesław Stręk

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