Circulating endothelial microparticles in patients with acute myocardial infarction
Abstract
Aim: To assess the presence of EMP in patients with acute MI in relation to early clinical outcome and coronary angiography results.
Materials and methods: EMPs counts were determined in 66 patients pts (23 women, 43 men) with documented ST elevation AMI and in 10 control patients with no evidence of coronary artery disease. All pts with AMI underwent coronary angiography with attempted primary angioplasty. EMPs were assayed by flow cytometry in platelet-poor plasma with combinations of fluorescent antibodies (anti CD31, -51, -42) allowing distinction of EMPs from platelet microparticles. Clinical and angiography results were compared with EMP levels.
Results: Three kinds of EMPs were measured: CD31+, CD51+ and CD31+/51+. The percentage of EMPs CD31+/CD51 was significantly (p=0,042) higher in patients with AMI in comparison with control subjects. However, a marker, which distinguished both groups the most, was the level of EMPs CD51+. It was significantly (p=0,024) higher in pts with AMI than in control pts. The levels of CD31+ were similar in both groups. There was no correlation between EMP levels, clinical and angiography results.
Conclusion: The presence of circulating EMPs provides direct evidence of endothelial injury in AMI. The clinical and practical value of these results, however, needs further exploration.
Abstract
Aim: To assess the presence of EMP in patients with acute MI in relation to early clinical outcome and coronary angiography results.
Materials and methods: EMPs counts were determined in 66 patients pts (23 women, 43 men) with documented ST elevation AMI and in 10 control patients with no evidence of coronary artery disease. All pts with AMI underwent coronary angiography with attempted primary angioplasty. EMPs were assayed by flow cytometry in platelet-poor plasma with combinations of fluorescent antibodies (anti CD31, -51, -42) allowing distinction of EMPs from platelet microparticles. Clinical and angiography results were compared with EMP levels.
Results: Three kinds of EMPs were measured: CD31+, CD51+ and CD31+/51+. The percentage of EMPs CD31+/CD51 was significantly (p=0,042) higher in patients with AMI in comparison with control subjects. However, a marker, which distinguished both groups the most, was the level of EMPs CD51+. It was significantly (p=0,024) higher in pts with AMI than in control pts. The levels of CD31+ were similar in both groups. There was no correlation between EMP levels, clinical and angiography results.
Conclusion: The presence of circulating EMPs provides direct evidence of endothelial injury in AMI. The clinical and practical value of these results, however, needs further exploration.
Keywords
acute myocardial infarction - endothelial microparticles- adhesive molecules


Title
Circulating endothelial microparticles in patients with acute myocardial infarction
Journal
Kardiologia Polska (Polish Heart Journal)
Issue
Pages
537-542
Published online
2005-12-12
Bibliographic record
Kardiol Pol 2005;62(6):537-542.
Keywords
acute myocardial infarction - endothelial microparticles- adhesive molecules
Authors
Marzenna Zielińska
Włodzimierz Koniarek
Jan Goch
Barbara Cebula
Marzena Tybura
Tadeusz Robak
Piotr Smolewski