open access

Vol 66, No 10 (2008)
Other
Published online: 2008-10-27
Submitted: 2012-12-28
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Original article
Gene polymorphisms and antigen levels of matrix metalloproteinase-1 in type 2 diabetes mellitus coexisting with coronary heart disease

Józef Drzewoski, Agnieszka Śliwińska, Karolina Przybyłowska, Tomasz Śliwiński, Jacek Kasznicki, Monika Żurawska-Klis, Marcin Kosmalski, Ireneusz Majsterek
Kardiol Pol 2008;66(10):1042-1048.

open access

Vol 66, No 10 (2008)
Other
Published online: 2008-10-27
Submitted: 2012-12-28

Abstract


Background and aim: Diabetes mellitus is a major risk factor for coronary heart disease (CHD). Matrix metalloproteinases (MMPs) can play a pivotal role in the remodelling of extracellular matrix associated with the development of atherosclerosis. Therefore, the aim of the study was to compare the distribution of genotypes and frequency of alleles of two polymorphisms of the MMP-1 gene promoter, an A/G substitution and a 1G/2G insertion, in correlation with antigen levels of matrix metalloproteinase-1 (MMP-1) in type 2 diabetic patients with or without CHD as well as individuals with normal glucose level without CHD. Methods: Genotypes of 115 patients with type 2 diabetes mellitus (T2DM) and a subpopulation of 66 patients with coexisting CHD as well as 120 non-diabetic control subjects were determined by PCR-based restriction fragment length polymorphism (PCR-RFLP).
Results: We demonstrated that antigen levels of MMP-1 in the serum of diabetic patients were significantly higher than those of individuals with normal glucose metabolism (p <0.05). Elevated levels of MMP-1 positively correlated with CHD occurrence in T2DM patients (p <0.01). The distribution of genotypes revealed higher frequency of the 2G/2G polymorphism variant in diabetic patients with CHD [OR 5.76, 95% CI (1.24; 26.87)], thus suggesting its strong association with high level of MMP-1. In T2DM patients with coexisting CHD, a higher frequency of the 2G allele of 1G/2G [OR 1.74, CI 95% (1.01; 2.99)] and the G allele of A/G polymorphism [OR 2.15, 95% CI (1.22; 3.80)] was also found.
Conclusion: Our results suggest that type 2 diabetes mellitus is linked with elevated blood level of MMP-1, and polymorphisms of the promoter region of its gene might be associated with CHD.

Abstract


Background and aim: Diabetes mellitus is a major risk factor for coronary heart disease (CHD). Matrix metalloproteinases (MMPs) can play a pivotal role in the remodelling of extracellular matrix associated with the development of atherosclerosis. Therefore, the aim of the study was to compare the distribution of genotypes and frequency of alleles of two polymorphisms of the MMP-1 gene promoter, an A/G substitution and a 1G/2G insertion, in correlation with antigen levels of matrix metalloproteinase-1 (MMP-1) in type 2 diabetic patients with or without CHD as well as individuals with normal glucose level without CHD. Methods: Genotypes of 115 patients with type 2 diabetes mellitus (T2DM) and a subpopulation of 66 patients with coexisting CHD as well as 120 non-diabetic control subjects were determined by PCR-based restriction fragment length polymorphism (PCR-RFLP).
Results: We demonstrated that antigen levels of MMP-1 in the serum of diabetic patients were significantly higher than those of individuals with normal glucose metabolism (p <0.05). Elevated levels of MMP-1 positively correlated with CHD occurrence in T2DM patients (p <0.01). The distribution of genotypes revealed higher frequency of the 2G/2G polymorphism variant in diabetic patients with CHD [OR 5.76, 95% CI (1.24; 26.87)], thus suggesting its strong association with high level of MMP-1. In T2DM patients with coexisting CHD, a higher frequency of the 2G allele of 1G/2G [OR 1.74, CI 95% (1.01; 2.99)] and the G allele of A/G polymorphism [OR 2.15, 95% CI (1.22; 3.80)] was also found.
Conclusion: Our results suggest that type 2 diabetes mellitus is linked with elevated blood level of MMP-1, and polymorphisms of the promoter region of its gene might be associated with CHD.
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Keywords

type 2 diabetes mellitus; coronary heart disease; metalloproteinases; genetic polymorphism

About this article
Title

Original article
Gene polymorphisms and antigen levels of matrix metalloproteinase-1 in type 2 diabetes mellitus coexisting with coronary heart disease

Journal

Kardiologia Polska (Polish Heart Journal)

Issue

Vol 66, No 10 (2008)

Pages

1042-1048

Published online

2008-10-27

Bibliographic record

Kardiol Pol 2008;66(10):1042-1048.

Keywords

type 2 diabetes mellitus
coronary heart disease
metalloproteinases
genetic polymorphism

Authors

Józef Drzewoski
Agnieszka Śliwińska
Karolina Przybyłowska
Tomasz Śliwiński
Jacek Kasznicki
Monika Żurawska-Klis
Marcin Kosmalski
Ireneusz Majsterek

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